155807 recombinant protein Search Results


155807 recombinant protein  (R&D Systems)
96
R&D Systems 155807 recombinant protein
155807 Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/155807+recombinant+protein/Recombinant+Mouse+Wnt-3a+Protein/10__7554_slash_elife__104068-707-152-158
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recombinant proteins teklad global  (Fisher Bioreagents)
86
Fisher Bioreagents recombinant proteins teklad global
Recombinant Proteins Teklad Global, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated vector lab  (Vector Laboratories)
96
Vector Laboratories biotinylated vector lab
Biotinylated Vector Lab, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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9008s  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc 9008s
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anti-cd31-apc  (Becton Dickinson)
90
Becton Dickinson anti-cd31-apc
Anti Cd31 Apc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brdu mobu 1 santa cruz  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology brdu mobu 1 santa cruz
Figure 2. Two distinct types of behavior in the crypt (A) Schematics of Lrig1;mTmG fate mapping experiment. Tissue was collected 48, 72, and 96 h post labeling (4-OHT injection). (B) Representative images of top- and bottom-rooted Lrig1 clones at 48, 72, and 96 h. mG (green) and non-clone cells, mT (magenta), and DAPI (blue). Scale bar, 20 mm. (C and D) Lrig1 clone sizes over time, separated by position (top or bottom rooted). Points represent individual clones. Number of clones is shown below x axis, and boxplots indicating the lower and upper quartiles as well as medians. Statistical significance was tested with non-paired Mann-Whitney (p < 0.0001). (E) Schematics of <t>EdU/BrdU</t> double labeling experiment. (F) Representative images of crypts (top and bottom), 15 h after the EdU pulse. EdU (magenta), BrdU (yellow), DAPI (blue), and E-cadherin (green). Scale bar, 10 mm. (G) EdU+BrdU+ over EdU+ cells in bottom and top compartment. Point represents individual mice (averaged over four crypts). Plot shows means ± SD. (H) Schematic of the mathematical model. (I) Mean Lrig1 clone sizes of top- (red) and bottom-rooted (blue) clones. Lines show model predictions, and shaded areas indicate 95% confidence intervals. Experimental data are means ± SD. Model parameters are listed in Table S2.
Brdu Mobu 1 Santa Cruz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/155807+recombinant+protein/BrdU+Antibody/pm39793582-178-24-26
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polyclonal bio rad  (Bio-Rad)
93
Bio-Rad polyclonal bio rad
Figure 2. Two distinct types of behavior in the crypt (A) Schematics of Lrig1;mTmG fate mapping experiment. Tissue was collected 48, 72, and 96 h post labeling (4-OHT injection). (B) Representative images of top- and bottom-rooted Lrig1 clones at 48, 72, and 96 h. mG (green) and non-clone cells, mT (magenta), and DAPI (blue). Scale bar, 20 mm. (C and D) Lrig1 clone sizes over time, separated by position (top or bottom rooted). Points represent individual clones. Number of clones is shown below x axis, and boxplots indicating the lower and upper quartiles as well as medians. Statistical significance was tested with non-paired Mann-Whitney (p < 0.0001). (E) Schematics of <t>EdU/BrdU</t> double labeling experiment. (F) Representative images of crypts (top and bottom), 15 h after the EdU pulse. EdU (magenta), BrdU (yellow), DAPI (blue), and E-cadherin (green). Scale bar, 10 mm. (G) EdU+BrdU+ over EdU+ cells in bottom and top compartment. Point represents individual mice (averaged over four crypts). Plot shows means ± SD. (H) Schematic of the mathematical model. (I) Mean Lrig1 clone sizes of top- (red) and bottom-rooted (blue) clones. Lines show model predictions, and shaded areas indicate 95% confidence intervals. Experimental data are means ± SD. Model parameters are listed in Table S2.
Polyclonal Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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20e3 rabbit mab  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 20e3 rabbit mab
Figure 2. Two distinct types of behavior in the crypt (A) Schematics of Lrig1;mTmG fate mapping experiment. Tissue was collected 48, 72, and 96 h post labeling (4-OHT injection). (B) Representative images of top- and bottom-rooted Lrig1 clones at 48, 72, and 96 h. mG (green) and non-clone cells, mT (magenta), and DAPI (blue). Scale bar, 20 mm. (C and D) Lrig1 clone sizes over time, separated by position (top or bottom rooted). Points represent individual clones. Number of clones is shown below x axis, and boxplots indicating the lower and upper quartiles as well as medians. Statistical significance was tested with non-paired Mann-Whitney (p < 0.0001). (E) Schematics of <t>EdU/BrdU</t> double labeling experiment. (F) Representative images of crypts (top and bottom), 15 h after the EdU pulse. EdU (magenta), BrdU (yellow), DAPI (blue), and E-cadherin (green). Scale bar, 10 mm. (G) EdU+BrdU+ over EdU+ cells in bottom and top compartment. Point represents individual mice (averaged over four crypts). Plot shows means ± SD. (H) Schematic of the mathematical model. (I) Mean Lrig1 clone sizes of top- (red) and bottom-rooted (blue) clones. Lines show model predictions, and shaded areas indicate 95% confidence intervals. Experimental data are means ± SD. Model parameters are listed in Table S2.
20e3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/155807+recombinant+protein/Phospho-Histone+H2A%2EX+(Ser139)+Rabbit+mAb/pm34813734-353-139-142
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Image Search Results


Figure 2. Two distinct types of behavior in the crypt (A) Schematics of Lrig1;mTmG fate mapping experiment. Tissue was collected 48, 72, and 96 h post labeling (4-OHT injection). (B) Representative images of top- and bottom-rooted Lrig1 clones at 48, 72, and 96 h. mG (green) and non-clone cells, mT (magenta), and DAPI (blue). Scale bar, 20 mm. (C and D) Lrig1 clone sizes over time, separated by position (top or bottom rooted). Points represent individual clones. Number of clones is shown below x axis, and boxplots indicating the lower and upper quartiles as well as medians. Statistical significance was tested with non-paired Mann-Whitney (p < 0.0001). (E) Schematics of EdU/BrdU double labeling experiment. (F) Representative images of crypts (top and bottom), 15 h after the EdU pulse. EdU (magenta), BrdU (yellow), DAPI (blue), and E-cadherin (green). Scale bar, 10 mm. (G) EdU+BrdU+ over EdU+ cells in bottom and top compartment. Point represents individual mice (averaged over four crypts). Plot shows means ± SD. (H) Schematic of the mathematical model. (I) Mean Lrig1 clone sizes of top- (red) and bottom-rooted (blue) clones. Lines show model predictions, and shaded areas indicate 95% confidence intervals. Experimental data are means ± SD. Model parameters are listed in Table S2.

Journal: Developmental cell

Article Title: Fate mapping in mouse demonstrates early secretory differentiation directly from Lgr5+ intestinal stem cells.

doi: 10.1016/j.devcel.2024.12.023

Figure Lengend Snippet: Figure 2. Two distinct types of behavior in the crypt (A) Schematics of Lrig1;mTmG fate mapping experiment. Tissue was collected 48, 72, and 96 h post labeling (4-OHT injection). (B) Representative images of top- and bottom-rooted Lrig1 clones at 48, 72, and 96 h. mG (green) and non-clone cells, mT (magenta), and DAPI (blue). Scale bar, 20 mm. (C and D) Lrig1 clone sizes over time, separated by position (top or bottom rooted). Points represent individual clones. Number of clones is shown below x axis, and boxplots indicating the lower and upper quartiles as well as medians. Statistical significance was tested with non-paired Mann-Whitney (p < 0.0001). (E) Schematics of EdU/BrdU double labeling experiment. (F) Representative images of crypts (top and bottom), 15 h after the EdU pulse. EdU (magenta), BrdU (yellow), DAPI (blue), and E-cadherin (green). Scale bar, 10 mm. (G) EdU+BrdU+ over EdU+ cells in bottom and top compartment. Point represents individual mice (averaged over four crypts). Plot shows means ± SD. (H) Schematic of the mathematical model. (I) Mean Lrig1 clone sizes of top- (red) and bottom-rooted (blue) clones. Lines show model predictions, and shaded areas indicate 95% confidence intervals. Experimental data are means ± SD. Model parameters are listed in Table S2.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti Human Lysozyme EC 3.2.1.17 (Concentrate) Agilent Dako Cat #: A0099; RRID: AB_2341230 Mouse Monoclonal anti BrdU (MoBU-1) Santa Cruz Cat #: sc-51514; RRID: AB_626519 GFP polyclonal antibody ThermoFisher Cat #: a11122; RRID: AB_221569 Alexa Fluor 647 Donkey anti Rabbit IgG (H+L) ThermoFisher Scientific Cat #: A-31573; RRID: AB_2536183 Alexa Fluor 555 Goat anti Mouse IgG2a (H+L) ThermoFisher Scientific Cat #: A-21137; RRID: AB_2535776 Anti Mouse EpCAM APC (G8.8) eBioscience Cat #: 17-5791-80, RRID: AB_1659714 PE-Cy7 Rat Anti Mouse CD31 BD biosciences Cat #: 561410; RRID: AB_10612003 PE-Cy7 Rat Anti Mouse CD45 BD biosciences Cat #: 552848; RRID: AB_394489 TotalSeq-A0301 anti-mouse Hashtag 1 Antibody Biolegend Cat #: 155801; RRID: AB_2750032 TotalSeq-A0302 anti-mouse Hashtag 2 Antibody Biolegend Cat #: 155803; RRID: AB_2750033 TotalSeq-A0303 anti-mouse Hashtag 3 Antibody Biolegend Cat #: 155805; RRID: AB_2750034 TotalSeq-A0304 anti-mouse Hashtag 4 Antibody Biolegend Cat #: 155807; RRID: AB_2750035 TotalSeq-A0305 anti-mouse Hashtag 5 Antibody Biolegend Cat #: 155809; RRID: AB_2750036 TotalSeq-A0306 anti-mouse Hashtag 6 Antibody Biolegend Cat #: 155811; RRID: AB_2750037 TotalSeq-A0307 anti-mouse Hashtag 7 Antibody Biolegend Cat #: 155813; RRID: AB_2750039 TotalSeq-A0308 anti-mouse Hashtag 8 Antibody Biolegend Cat #: 155815; RRID: AB_2750040 Chemicals, peptides, and recombinant proteins 4-hydroxytamoxifen Sigma-Aldrich Cat #: H6278 EdU (5-ethynyl-20-deoxyuridine) Invitrogen / Thermo Fisher Scientific Cat #: A10044 BrdU (5-Bromo-20-Deoxyuridine) Invitrogen Cat #: 11594167 N,N,N0,N0-Tetrakis(2-hydroxypropyl)ethylenediamine Sigma-Aldrich Cat #: 122262 Urea Sigma-Aldrich Cat #: U5378 Triton x-100 Sigma-Aldrich Cat #: X100 Bovine Serum Albumin Sigma-Aldrich Cat #: A9576 RapiClear 1.52 Sunjin Lab Cat #: RC152002 Normal Goat Serum Sigma-Aldrich Cat #: G9023 HBSS Ca2+Mg2+ free ThermoFischer Scientific Cat #: 14175095 UltraPure 0.5M EDTA Invitrogen Cat #: 15575-020 HEPES buffer Gibco/Life Technologies Cat #: 15630-056 TrypLE Express ThermoFisher Scientific Cat #: 12605036 Y-27632 dihydrochloride Sigma-Aldrich Cat #: Y0503 N-Acetyl-L-cysteine Sigma-Aldrich Cat #: A9165 DNaseI Roche Cat #: 10104159001 aDMEM/F12 Gibco/Life Technologies Cat #: 12634-028 0.1%BSA PBS Sigma-Aldrich Cat #: A4503-100 DAPI Sigma-Aldrich Cat #: D9542 (Continued on next page) e1 Developmental Cell 60, 1–9.e1–e6, May 5, 2025

Techniques: Labeling, Injection, Clone Assay, MANN-WHITNEY